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KMID : 0364820130490010038
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Korean Journal of Microbiology
2013 Volume.49 No. 1 p.38 ~ p.45
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Characterization and Organic Hydrocarbons Degradation Potential of Euryhaline Marine Microorganism, Bacillus sp. EBW4 Isolated from Polychaete (Perinereis aibuhitensis)
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Shin Se-Yeon
Khorloo Yundendorj
Lee Sang-Suk
Lee Dong-Heon
Kang Kyung-Ho
Kang Hyung-Yeel
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Abstract
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In this study, euryhaline marine microorganism, Bacillus sp. strain EBW4 isolated from polychaete (Perinereis
aibuhitensis) of Suncheon Bay was physiologically, biochemically and genetically characterized. Based on 16S
rRNA sequence, EBW14 was found to share 98.25% similarity with Bacillus hemicentroti JSM076093T, 97.96%
similarity with Bacillus hwajinponensis SW-72T and 96.28% similarity with B. algicoa KMM3737T , respectively.
The temperature range for the growth of strain EBW4 was 4?40℃, NaCl concentration range 0?17% and pH range
pH 5?9, revealing that EBW4 was euryhaline bacterium. Major fatty acids in strain EBW4 were composed of anteiso
C15:0 (48.2%), iso C16:0 (12.1%), anteiso C17:0 (11.6%) and iso C14:0 (9.4%). EBW4 was found to have DNase,
amylase, protease and lipase for the degradation of macromolecules such as DNA, carbohydrates, proteins, lipids,
etc. The enzyme activities of alkaline phosphatase, esterase (C4), leucine arylamidase and α-chymotrypsin were also
found in strain EBW4. Analysis of the biodegradation ability of EBW4 for organic hydrocarbons under different salinity conditions using synthetic water waste revealed that EBW4 exhibited the ability to degrade organic hydrocarbons very quickly, suggesting strain EBW4 may be a good candidate for the application to various
industries.
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KEYWORD
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Bacillus, Perinereis aibuhitensis, euryhaline microorganism, organic hydrocarbons degradation
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